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Laboratory methods in enzymology : cell, lipid and carbohydrate
Enregistré dans:
| Autres auteurs : | |
|---|---|
| Format : | Livre |
| Langue : | anglais |
| Titre complet : | Laboratory methods in enzymology : cell, lipid and carbohydrate / edited by Jon Lorsch |
| Publié : |
Amsterdam :
Elsevier B. V.
, 2013, cop. 2013 |
| Collection : | Methods in enzymology (Online) ; 533 |
| Accès en ligne : |
Accès Nantes Université
|
| Sujets : | |
| Documents associés : | Autre format:
Laboratory methods in enzymology |
- P. 3
- Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler
- P. 15
- Storage of bacteria and yeast / Cheryl M. Koh
- P. 25
- Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen
- . P 31
- Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen
- P. 43
- Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson
- P. 57
- Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles
- P. 79
- Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court
- P. 103
- Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici
- P. 133
- Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart
- P. 157
- Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court
- P. 181
- Growth media for E. coli / Juliane C. Lessard
- P. 191
- Saccharomyces cerevisiae growth media / Jessica S. Dymond
- P. 207
- Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman
- P. 217
- Preparation of slides for microscopy from frozen tissue sections / Julia Bachman
- P. 225
- Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams
- P. 235
- Preparation of cells for microscopy using cytospin / Cheryl M. Koh
- P. 241
- Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh
- P. 249
- Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh
- P. 259
- Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy
- P. 267
- Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak
- P. 275
- Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak
- P. 283
- Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak
- P. 291
- Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams
- P. 303
- Thin layer chromatography / Marina Santiago and Scott Strobel
- P. 325
- ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch