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|a Laboratory Methods in Enzymology
|b Ressource électronique
|e RNA
|f edited by Jon Lorsch
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|a Amsterdam
|c Elsevier B. V.
|d 2013, cop. 2013
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|a Methods in enzymology
|v 530
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|a Livre électronique. Données textuelles
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|a Description d'après la consultation du 2013-10-18
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|a L'impression du document génère 413 p.
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|a Version électronique de l'édition de : San Diego, CA : Academic Press, 2013
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|a L'accès à cette ressource est réservé aux usagers des établissements qui en ont fait l'acquisition
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|a Réf. bibliogr. en fin de chapitres. Index
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|a 30 fichiers au format HTML et au format PDF
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|a Configuration requise : navigateur internet et lecteur de fichiers PDF
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|b 1. Computational prediction of RNA secondary structure
|b 2. Reverse-transcription PCR (RT-PCR)
|b 3. Northern blotting
|b 4. Explanatory chapter: nuclease protection assays
|b 5. In vitro transcription from plasmid or PCR-amplified DNA
|b 6. Measurement of in vivo synthesis rates
|b 7. Method for measuring mRNA decay rate in saccharomyces cerevisiae
|b 8. Analysis of polysomes from bacteria
|b 9. Polysome profile analysis - yeast
|b 10. Polysome analysis of mammalian cells
|b 11. Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae
|b 12. Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates
|b 13. Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA
|b 14. RNA radiolabeling
|b 15. Fluorescently labeling synthetic RNAs
|b 16. Analysis of RNA by analytical polyacrylamide gel
|b 17. RNA purification by preparative polyacrylamide gel
|b 18. Explanatory chapter: nucleic acid concentration determination
|b 19. RNA purification-precipitation methods
|b 20. Reverse transcriptase dideoxy sequencing of RNA
|b 21. RNA structure experimental analysis-chemical modification
|b 22. Structural analysis of RNA backbone using in-line probing
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|0 104870729
|t Methods in enzymology (Online)
|x 1557-7988
|v 530
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452 |
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| |
|0 172782023
|t Laboratory Methods in Enzymology
|b Texte imprimé
|o RNA
|f edited by Jon Lorsch,...
|c Amsterdam
|n Elsevier/ Academic Press
|d 2013, cop. 2013
|p 1 vol. (lvii-413 p.)
|s Methods in enzymology
|y 978-0-12-420037-1
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