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Cell Separation : Fundamentals, Analytical and Preparative Methods
Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biolog...
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| Autres auteurs : | , |
| Format : | Livre |
| Langue : | anglais |
| Titre complet : | Cell Separation : Fundamentals, Analytical and Preparative Methods / edited by Ashok Kumar, Igor Yu Galaev, Bo Mattiasson. |
| Édition : | 1st ed. 2007. |
| Publié : |
Berlin, Heidelberg :
Springer Berlin Heidelberg
, [20..] Cham : Springer Nature |
| Collection : | Advances in biochemical engineering, biotechnology (Internet) ; 106 |
| Titre de l'ensemble : | Advances in Biochemical Engineering/Biotechnology vol. 106 |
| Accès en ligne : |
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| Condition d'utilisation et de reproduction : | Conditions particulières de réutilisation pour les bénéficiaires des licences nationales : https://www.licencesnationales.fr/springer-nature-ebooks-contrat-licence-ln-2017 |
| Contenu : | Methods in Cell Separations. Flow Cytometry and Cell Sorting. Cell Isolation and Expansion Using Dynabeads ®. Affinity Adsorption of Cells to Surfaces and Strategies for Cell Detachment. Chromatography of Living Cells Using Supermacroporous Hydrogels, Cryogels. Hollow-Fibre Affinity Cell Separation. Cell Partitioning in Aqueous Two-Phase Polymer Systems. Development of Separation Technique for Stem Cells |
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| Documents associés : | Autre format:
Cell separation |
| Résumé : | Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biological applications has increased. Ef?cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci?c separation of a discrete population of cells from a mixture. For example, in the ?eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi?cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells,regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup |
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| Variantes de titre : | With contributions by numerous experts |
| ISBN : | 978-3-540-75263-9 |
| DOI : | 10.1007/978-3-540-75263-9 |